Cultivation and Differentiation of Encapsulated hMSC-TERT in a Disposable Small-Scale Syringe-Like Fixed Bed Reactor

نویسندگان

  • Christian Weber
  • Sebastian Pohl
  • Ralf Pörtner
  • Christine Wallrapp
  • Moustapha Kassem
  • Peter Geigle
  • Peter Czermak
چکیده

The use of commercially available plastic syringes is introduced as disposable small-scale fixed bed bioreactors for the cultivation of implantable therapeutic cell systems on the basis of an alginate-encapsulated human mesenchymal stem cell line. The system introduced is fitted with a noninvasive oxygen sensor for the continuous monitoring of the cultivation process. Fixed bed bioreactors offer advantages in comparison to other systems due to their ease of automation and online monitoring capability during the cultivation process. These benefits combined with the advantage of single-use make the fixed bed reactor an interesting option for GMP processes. The cultivation of the encapsulated cells in the fixed bed bioreactor system offered vitalities and adipogenic differentiation similar to well-mixed suspension cultures.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Mathematical modeling of a fixed bed chromatographic reactor for Fischer Tropsch synthesis

In this research, Fischer Tropsch synthesis (FTS) has been modeled in the fixed bed chromatographic reactor for the first time by applying a rather complex dispersed plug flow model for fluid phase and linear driving force (LDF) model for adsorbent. Model equations are dynamic, multi-component, non-linear and heterogeneous including reaction and adsorption simultaneously Complex kinetics fo...

متن کامل

Enzymatic Detachment of Therapeutic Mesenchymal Stromal Cells Grown on Glass Carriers in a Bioreactor

Cell therapies require the in vitro expansion of adherent cells such as mesenchymal stromal cells (hMSCs) in bioreactor systems or other culture environments, followed by cell harvest. As hMSCs are strictly adherent cells, cell harvest requires cell detachment. The use of hMSCs for cell therapy requires GMP production in accordance with the guidelines for advanced therapeutic medical products. ...

متن کامل

V2O5-MoO3 Catalysts Preparation and Performance Testing on the Oxidation of Benzene to Maleic Anhydride in a Fixed Bed Reactor

In this research, the selective oxidation of benzene to maleic anhydride (MAN) in a fixed-bed reactor was studied. The gas phase catalytic oxidation of benzene was carried outin a laboratory scale fixed bed reactor on the catalysts, which were prepared having two different compositions on silica gel. The effects of temperature, flow rates of benzene and air, and catalyst type on the reactio...

متن کامل

Expansion and Harvesting of hMSC-TERT

The expansion of human mesenchymal stem cells as suspension culture by means of spinner flasks and microcarriers, compared to the cultivation in tissue culture flasks, offers the advantage of reducing the requirements of large incubator capacities as well as reducing the handling effort during cultivation and harvesting. Nonporous microcarriers are preferable when the cells need to be kept in v...

متن کامل

Effect of the chemical nature of fixed-bed reactor support materials on bioreactor performance and biomass accumulation

This study investigated the effect, on reactor performance and biomass retention inside the bed, of the material used to make the supports of anaerobic fixed-bed reactors. Three inert supports of similar shape but made of three different materials polyvinyl chloride, polypropylene, high-density polyethylene were manufactured and used. All three supports had the same specific surface area but di...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 1  شماره 

صفحات  -

تاریخ انتشار 2007